Review



pbabe puro vectors carrying erbb2 wild type  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc pbabe puro vectors carrying erbb2 wild type
    (A) MCF10a cells were transduced with different <t>ErbB2</t> variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.
    Pbabe Puro Vectors Carrying Erbb2 Wild Type, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+vectors+carrying+erbb2+wild+type/bio_rxiv__2024__11__19__624272-173-5-17?v=Addgene+inc
    Average 93 stars, based on 34 article reviews
    pbabe puro vectors carrying erbb2 wild type - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis"

    Article Title: Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis

    Journal: bioRxiv

    doi: 10.1101/2024.11.19.624272

    (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.
    Figure Legend Snippet: (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.

    Techniques Used: Transduction, Cell Culture, Co-Culture Assay, Isolation, Staining, Expressing, Mutagenesis



    Similar Products

    pbabe puro vectors carrying erbb2 wild type  (Addgene inc)
    93
    Addgene inc pbabe puro vectors carrying erbb2 wild type
    (A) MCF10a cells were transduced with different <t>ErbB2</t> variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.
    Pbabe Puro Vectors Carrying Erbb2 Wild Type, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+vectors+carrying+erbb2+wild+type/bio_rxiv__2024__11__19__624272-173-5-17?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    pbabe puro vectors carrying erbb2 wild type - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.

    Journal: bioRxiv

    Article Title: Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis

    doi: 10.1101/2024.11.19.624272

    Figure Lengend Snippet: (A) MCF10a cells were transduced with different ErbB2 variants and co-cultured with either Vγ9Vδ2TCR or HER2-CAR transduced T cells. Tumor cells were pre-treated with the PI3K kinase inhibitor Pictilisib at 2uM overnight. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (B) Furthermore, tumor cells were isolated and stained for BTN2A1 via TCR tetramer staining and (C) BTN3A cell surface expression. (D) Protein expression of HER2, phosphorylated AKT (pAKT) and total AKT in MCF10a mutant lines cultured for 24h in full culture medium (F) or medium without additional growth factors (S) . (E) Multiple tumor cell lines were co-cultured with Vγ9Vδ2TCR T-cells after pre-treatment with either the PI3K kinase inhibitor, AKT inhibitor or MEK inhibitor. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (F) Multiple tumor cell lines were pre-treated with either PI3K kinase inhibitor, AKT inhibitor, mTOR inhibitor Rapamycin or mTOR inhibitor Torin1 and subsequently co-cultured with Vγ9Vδ2TCR T cells. After an overnight co-culture, supernatant was used to determine IFNγ production by the Vγ9Vδ2TCR T cells. (G) Heatmap illustrating the expression of genes from the ‘PI3K_and_AKT_family’ gene list, in the WT-AKPS model, with or without PAM. (H) Heatmap of Pearson’s correlation of the expression of genes from the ‘PI3K_and_AKT_family’ and BTNx genes in the WT-AKPS model, with or without PAM.

    Article Snippet: pBABE-Puro retroviral vector (EV) and pBABE-Puro vectors carrying ERBB2 wild-type and mutants were co-transfected independently with pUMVC (Addgene #8449) and VSV-G (Addgene #8454) retroviral packaging plasmids into HEK-293T cells using PEI-transfection.

    Techniques: Transduction, Cell Culture, Co-Culture Assay, Isolation, Staining, Expressing, Mutagenesis